ELISA EC50 binding affinity of select VNAR-hFc antibodiesto Wuhan and mutant spike proteins. ELISA was used to determine EC50 values for individual VNAR-hFc antibodies to (A) S1, (B) S1-RBD, (C) S1- RBD N501Y, and (D) S1-RBD E484K. Microplates were coated with different spike proteins and incubated with serially diluted VNAR-hFc antibodies followed by detection with HRP conjugated anti-human Fc antibody. The developed plates were read at 450nm and OD was used for 4-parametric non-linear regression model to calculate EC50 values.
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3.23 FAQ-251 How to compute EC50/IC50 in Dose Response fitting
t_kahi’s blog IC50/EC50値の算出について:Assay Operations for SAR Support in Assay Guidance Manual
Proteintech TNF alpha (HZ-1014) demonstrates equivalent induction cytotoxicity and EC50 to leading competitors. TNF alpha demonstrates does-dependent cytotoxicity in the L929 mouse adipose cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. L929 cells were treated with increasing concentrations of GMP recombinant TNF alpha for 72 hours in the presence of actinomycin D. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.002-0.026 ng/mL.