ELISA EC50 binding affinity of select VNAR-hFc antibodiesto Wuhan and mutant spike proteins. ELISA was used to determine EC50 values for individual VNAR-hFc antibodies to (A) S1, (B) S1-RBD, (C) S1- RBD N501Y, and (D) S1-RBD E484K. Microplates were coated with different spike proteins and incubated with serially diluted VNAR-hFc antibodies followed by detection with HRP conjugated anti-human Fc antibody. The developed plates were read at 450nm and OD was used for 4-parametric non-linear regression model to calculate EC50 values.
Euroemme® EC50 Cone fan
3.23 FAQ-251 How to compute EC50/IC50 in Dose Response fitting