Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Alcohol Dehydrogenase antibody (ET7108-64) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7108-64) at 1/50 dilution for 0.5 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Alcohol Dehydrogenase 1A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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