Commercial ligases are supplied with a reaction buffer containing atp and mg2+, which are both firstly the dna ends have to collide by chance and stay together long enough for the ligase to join. What is the role of DNA helicase, DNA polymerase and DNA ...
In semiconservative DNA replication an existing DNA molecule is separated into two template strands. New nucleotides align with and bind to the nucleotides of the existing strands, thus forming two DNA molecules that are identical to the original DNA molecule.
Dna分子構造
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Part A shows an illustration of a DNA double helix, which has a sugar phosphate backbone on the outside and nitrogenous base pairs on the inside. Part B shows base-pairing between thymine and adenine, which form two hydrogen bonds, and between guanine and cytosine, which form three hydrogen bonds.