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DDIT3 - DNA damage-inducible transcript 3

DDIT3 Monoclonal Antibody (OTI4C4), TrueMAB™DDIT3 Monoclonal Antibody (OTI2D3), TrueMAB™DDIT3 antibodyXL DDIT3 BAXL DDIT3 BADescriptionClinical DetailsImagesExpected PatternsLiteratureDownloadsRelated PublicationsRelated NewsImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDIT3 (phospho S30) antibody (ab63392)Flow cytometric analysis of HepG2 cells with DDIT3 antibody at 1/50 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Western blot analysis of DDIT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-05, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: LOVO cell lysateLane 2: PC-12 cell lysatePredicted band size: 19 kDaObserved band size: 25 kDa
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